effect of dimethylnitrosamine on the activities of renal puenolsulfotransferase and arylsulfatases A and B in schistosoma mansoni infected mice

The activities of renal phenolsulfotransferase and arylsulfatases A and B were estimated in 400 male Swiss albino mice classified into four groups: Normal controls, Schistosoma mansoni infected group, Dimethylnitrosamine [DMN] treated group and infected treated group. The activity levels of the studied enzymes were significantly increased in all groups when compared with the control group, also the statistical analyses showed a high significant increase of the three enzymes levels in the infected treated group; when compared separately with treated or infected groups. It was concluded, therefore, that schistosomal infection is implicated in the development of kidney cancer which may arise from the pattern of hepatic mixed-function oxidase induction characterized for schiatosomiasis and its temporal relationship with the procarcinogenic initiating events. Furthermore, the striking significant increase in the enzymatic activity levels of the acid hydrolases arylsulfatases due to the lesion of both cytotoxic effects of dimethylnitrosamine as well as pathological change of schistosomiasis which may play an active role in the initiation of the malignant process by detoxifying endogenous sulfated aromatic metabolites

Glutathione content and glutathione -S-transferase activity in urinary bladder tumor tissues associated with schistosomiasis

GSH and GST activity were measured in eleven human bladder tumor and adjacent uninvolved specimens obtained from Egyptian patients with a history of schistosomal infection, undergoing cystectomy. The expression of GST[pi] was also measured using Western blotting analysis. GSH was higher in the tumor than in surrounding uninvolved tissue, though the difference was not significant. Total GST activity and GST[pi] expression were significantly higher [p < 0.05] in tumor tissues than in uninvolved tissues. There was a positive correlation between GST activity and GSH content in tumor tissues [r=0.64, p < 0.0005] and in surrounding uninvolved tissues [r = 0.72, p < 0.001]. A good correlation was also observed between total GST activity and GST[pi]expression in both tumor [r = 0.75, p < 0.05] and uninvolved tissues [r = 0.86, p<0.01]. To our knowledge, this is the first report of GST activity and GSH content in bladder cancer tissues associated with schistosomiasis. Our results suggest that one mechanism of resistance of this tumor to DNA interacting agents might be related to their inactivation by the GSH/GST system